LOCUS       40924_38213        5241 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pRSG43 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Yamamoto K, Nishimura M, Kato D, Takeo M, Negoro S.
  TITLE     Identification and characterization of another 4-nitrophenol 
            degradation gene cluster, nps, in Rhodococcus sp. strain PN1
  JOURNAL   J. Biosci. Bioeng. 111 (6), 687-694 (2011)
  PUBMED    21396889
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Takeo M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2009) Contact:Masahiro Takeo University of Hyogo, 
            Graduate School of Engineering, Dept. of Materials Science and 
            Chemistry; 2167 Shosha, Himeji, Hyogo 671-2280, Japan
REFERENCE   3  (bases 1 to 5241)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5241)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biosci. 
            Bioeng."; date: "2011"; volume: "111"; issue: "6"; pages: "687-694"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-SEP-2009) Contact:Masahiro Takeo University of Hyogo, Graduate 
            School of Engineering, Dept. of Materials Science and Chemistry; 
            2167 Shosha, Himeji, Hyogo 671-2280, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             327..1139
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1361..1377
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1378..1434
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1447..1463)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1471..1487)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1495..1525)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1540..1561)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             3065..3985
                     /codon_start=1
                     /gene="repA"
                     /product="replicase"
                     /label=repA
                     /protein_id="BAI39644.1"
                     /translation="MDMSGGSLSGDWEQLWLPLWPLATDDLLLGVYRMPRQDALDRRYL
                     EANPQALSNLLVVDVDHPDAALRALSAAGNHPLPNAIVENPRNGHAHAVWALTEPFTRT
                     EYARRKPLAYAAAVNEGLRRAVDGDAAYSGLMTKNPTHSAWDTHWIHAETRSLADLEHD
                     LGKHMPPPRWRQSKRRREDPVGLGRNCMLFETARTWAYRELRCHWGDPEGLGKAIQVEA
                     ADLNAAFSEPLPVSEVRAIAASIHRWIVTKSRMWADGPAVYEATFVAIQSARGRKMTEK
                     KREANRRRATKYDRDLVRKEATDGS"
     gene            3065..3985
                     /gene="repA"
                     /label=repA
     CDS             3975..4256
                     /codon_start=1
                     /gene="repB"
                     /product="DNA-binding replication protein"
                     /label=repB
                     /protein_id="BAI39645.1"
                     /translation="MGAETPARRTRTAREVAERIGASPRTVRRIIAEPRASYEARAAER
                     RKQVLELRASGMKLREIAAEVGMSVGGVGTILHHARKTEQSKAEGAMA"
     gene            3975..4256
                     /gene="repB"
                     /label=repB
     rep_origin      complement(4598..5186)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca ggcctgaatc gccccatcat
       61 ccagccagaa agtgagggag ccacggttga tgagagcttt gttgtaggtg gaccagttgg
      121 tgattttgaa cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct
      181 gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg tctcaaaatc
      241 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
      301 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga
      361 agccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
      421 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
      481 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
      541 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
      601 atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag
      661 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
      721 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
      781 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
      841 gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt
      901 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
      961 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     1021 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     1081 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     1141 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     1201 gcggctttgt tgaataaatc gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
     1261 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     1321 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     1381 ttcgagctcg gtacccgggg atcctctaga gtcgacctgc aggcatgcaa gcttggcgta
     1441 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     1501 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     1561 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     1621 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt ggcgaacttt tgctgagttg
     1681 aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc
     1741 aaaatcagta accgtcagtg ccgataagtt caaagttaaa cctggtgttg ataccaacat
     1801 tgaaacgctg atcgaaaacg cgctgaaaaa cgctgctgaa tgtgcgagct tcttccgctt
     1861 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1921 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgga
     1981 ggtgacgcga atgcatgaag catgtcgcac ttgcgcgcct tgtcctgtta tctgtaagat
     2041 cgacccctgg tgtacctcgt gcacccaaaa tcaggcccgg tggttccttt ggacccgggc
     2101 cttcgttatt tccacgccag cccgagctcc gcccgctcgt gcaagcgtca tgctcttcgt
     2161 ccgtgatcaa gacctccgaa cctatggccg gccaaccccc tcgggttgcg tcggcctgac
     2221 ccgttctagg gcgtctacgc ggccgctttc ccactccgtc cataccaacc cccgcaccaa
     2281 aggtccgggg gttttttcat gcccggattc ggtcgcggct gcgcctcgac ggtctccggt
     2341 tgcccaagga ggcacccatg acttgctcca cctgctcctc gcctgccccc gaaccgcgac
     2401 cgtcgcgcaa agaggcggtc cagcaactcg cgatccgatc gctggcgttc ttgtttcgcc
     2461 actgcacacg aatcgccctg aacgaagtgg tccaagaact gatccgcatc aagttcggcg
     2521 gttgaccgcg gacgtgcacc tgtagagcgg gttgcagcga gacaccgatg aaccactctc
     2581 cgctgcctag gcgacccggt tctggaaaga tcatcaccga gtgtccggcc ccaccccctg
     2641 cgggccggac actcatctgt atggcagcgt gcctcccttc ctgcccttcc cactgatcgt
     2701 ttcctcctgc caaaaatcgg gacacacctc ttgcagaagt tctgacaccc gggaaaggcc
     2761 ggccgaaagg gggcgctcac cgaccactct gatcgagaag ttctgccgca cccaccagcc
     2821 gtacccggcc aaccttccgc agtcccagcc gtacgaaacg gtctcgtgcc actccaccgg
     2881 ccctggtgtc gatcgactac aaaccaagat ccccacacac ctcatgcact aaagctgcga
     2941 ccacgaagaa caaggtggtc cgggtaagac ggaagggagt tttcccagga gggtcgccga
     3001 aacatctgac ttggttggcg tgtcctacat aaaaaaattg atcttgcgtg tgagggtgtc
     3061 acgcatggat atgagcgggg gatctctcag tggggactgg gagcagttgt ggctgcctct
     3121 gtggccgctc gcaacggacg atttgttgct tggggtctac cggatgcctc gccaggatgc
     3181 gctcgatcgg cgctaccttg aggccaatcc gcaggcgctg agcaatctcc tcgtcgtcga
     3241 tgtcgatcat ccagacgcgg cactgcgggc tctgtctgcc gccggcaacc atcccttgcc
     3301 gaacgcgatc gtggaaaacc cgcgcaatgg acacgcacat gcggtgtggg cattgaccga
     3361 acctttcacg cgcaccgagt acgccagacg taagccactc gcttatgccg cagcggtaaa
     3421 cgaggggctg cgtcgagctg tcgatggcga tgccgcctat tcggggttga tgacgaagaa
     3481 cccgactcac tcagcctggg acacacactg gatccacgcc gagactcgat cgctggcaga
     3541 tctcgaacat gacctcggaa agcatatgcc gccaccccgg tggcgacaga gcaaacgtcg
     3601 tcgcgaagac ccagtcggac tcggacgtaa ttgcatgctc ttcgagacgg cacgcacttg
     3661 ggcataccgc gaattgcgtt gccattgggg agatcccgaa ggtttaggga aagcaattca
     3721 ggtcgaagcc gcagacctta acgctgcctt ctctgagcct ttgccggtaa gcgaagtacg
     3781 agctatcgca gccagcattc accgctggat cgtcaccaag tcccgcatgt gggccgatgg
     3841 ccctgcggtt tacgaagcca ccttcgtcgc tatccaatcc gctcgcggac gcaagatgac
     3901 ggagaagaag cgcgaggcca atcgtcgccg tgcaacgaag tacgaccgcg acctcgtgag
     3961 gaaggaggcg accgatggga gctgagacgc cggcccggcg aacccgcaca gctcgcgaag
     4021 tggcggaacg aatcggtgcg tccccacgca cggtgcggcg catcatcgcg gagcctcgag
     4081 cttcatacga agctcgagca gctgaacgtc gaaagcaagt actcgaactc cgtgcgagcg
     4141 ggatgaagct gcgtgagatc gcggcggagg taggtatgtc ggtcggtgga gtagggacga
     4201 tcctgcatca cgcccgtaag accgagcagt ctaaggctga aggagctatg gcatgaacga
     4261 cgccatatcg gcccgcatca ctgcaatgca ggcccaactg acggctgtac ataccgagct
     4321 acgtgctcta gcggagctgg tggacatgct tgatgccgac gctctcgatg ctgagaccga
     4381 agattcagtg cgcgaagtga tcgactccct ggcagacgct gggcgagctc tagctggcgc
     4441 cgacgagccg ctccaggccg caattcatca cgcccggcga ctgccttagt cagcttctgt
     4501 ccgatcgatg gcaagccacc gcgaagcggt ggcgcgacat gtgagcaaaa ggccagcaaa
     4561 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4621 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4681 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4741 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     4801 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4861 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4921 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4981 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     5041 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5101 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5161 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5221 ctcagtggaa cgatccgtcg a
//
